Knowledge-enriched characterization of cell populations. Morphological information and facts from photographs can increase to the richness of flow cytometry information. As an example, the determine reveals an usually standard apoptosis assay working with Annexin V and PI, adding mobile imaging to characterize cells in each population to reveal morphologically unique characteristics. These insights couldn't are attained from multiplex staining on your own.

Whether you’re a novice or a professional, investigate our sources to advance your investigation and abilities in flow cytometry.

(B) RAW 264.7 macrophages tend to be annotated inaccurately with regard to particle depend because of their complexity and noncircular borders. The amount of singlets obtainable for Evaluation boosts as soon as the person trains and employs a more exact model.

Find new insights into your experimental information by visualizing populations using combined fluorescence and morphology details. Our software package interface wizards enable downsampling, concatenation (merged information) and dimensionality equipment which might be built in to the software for an intuitive, streamlined facts analysis practical experience.

*Pricing is indicative only and is also subject to alter. You should Call your Agilent agent with any thoughts. The information established forth herein is just not a suggestion or perhaps a commitment, each of which happen to be matter to credit approval, terms and conditions, and availability with Agilent. Agilent Economic Alternatives is available in chosen nations.

On top of that, it is illuminating to view the quantity of doublets slip by conventional singlet gates, and wonderful to confirm the status of unpredicted double-positive gatherings in my Examination."

Pair your Attune flow cytometer having an autosampler for enhanced workflow efficiency, and incorporate robotic integration for optimum throughput.

Our scientists will style and design panels for here you as repeatedly you will need—gratis, no solution obtain wanted.

The Attune NxT cytometer could be the trustworthy workhorse instrument your lab can depend upon for constant and trusted overall performance. The Attune CytPix product contains a large-pace brightfield digicam and AI-driven impression Evaluation software package, with current updates which includes device Studying tools and an intuitive person interface, enabling enhanced data precision, precision, and more rapidly insights into sample biology.

Probably most importantly, these activities comprise cells of Plainly various phenotypes which may lead to incorrect conclusions relating to double optimistic functions (especially in exceptional populations).

AI-driven automated image analysis with the Attune Cytometric Software translates party options into distinct morphology parameters that may be combined with regular fluorescence and scatter parameters to reveal extremely reproducible population variations and distinctive subsets.

We Formerly shown the power of imaging Automobile-T/Ramos mobile interactions. Enable’s look at just the inhabitants of finest desire, the double constructive activities, To find out more. We can easily now use extended morphological parameters (below, circularity and skewness of intensity) to more look at the options of those populations and refine gating on these functions, expanding info robustness.

Built-in dimensionality reduction equipment improved resolution into populations. Fluorescently gated populations back again-gated on to UMAP plot to verify anticipated populace resolution in lysed full human blood. Consumer can check out events from each cluster for Visible confirmation of cell phenotypes.

No clean/no lyse leukocyte Evaluation with imaging. Cells have been obtained from 24-hour-old blood by dilution in 1 mM EDTA ( Expert UX/UI enables end users to educate AI products for expanded apps. (A) The “Teach Product” characteristic delivers annotation tools and authentic-time model education accuracy.

At the guts of each Attune Flow Cytometer lies our revolutionary acoustic concentrating technological innovation, which makes use of sound waves to meticulously align cells inside the flow cell. This revolutionary tactic surpasses regular hydrodynamic methods, resulting in more quickly processing speeds, exceptional knowledge good quality, and elevated sample throughput.

The Attune Xenith cytometer is usually a large-parameter spectral analyzer, giving the two spectral unmixing and standard flow abilities for in depth and versatile cell Examination.

The Attune CytPix Flow Cytometer will allow end users to visualize samples in the course of acquisition, validate the accuracy of gating strategies, and support success with reputable documentation for publication functions.

Right here, we show that by gating strategies using image-centered quantitative parameters can distinguish interacting cells from coincident functions to more accurately assess interacting cells.